Asian Journal of Research in Chemistry

M. M. Maste ¹, A. N. Parate ², A. R. Bhat ¹

Affiliations
1 Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, JNMC Campus, Nehrunagar, Belgaum-10, Karnataka, IN
2 Department of Pharmaceutical Analysis, Prin. K.M. Kundnani College of Pharmacy, Jote Joy Building, Cuffe Parade, Mumbai, IN

Abstract

A gradient LC method was developed for determination and quantitation of Sitagliptin in bulk drug and pharmaceutical formulations. The separation was accomplished on a symmetry reversed phase C18 column, 75 mm × 4.6mm I.D., 3.5μm column using mobile phase consist of acetonitrile and 0.03% formic acid at flow rate of 0.3ml/min. The eluents were monitored with a UV detector set at 268 nm as detection wavelength. The investigated validation elements showed the method has acceptable specificity, accuracy, linearity, precision, robustness. This method was found to be linear within range of 100 to 500 μg.ml^-1 (r=0.9999). The method could be of use for rapid and routine quality control analysis of Sitagliptin. Thus, the developed method can be used for process development as well as quality assurance of Sitagliptin in bulk drug and pharmaceutical formulations.

Keywords

Sitagliptin Phosphate, RP-HPLC, Validation, Liquid Chromatography, Quality Assurance.

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M. M. Maste ¹, A. N. Parate ², A. R. Bhat ¹

Affiliations
1 Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, JNMC Campus, Nehrunagar, Belgaum-10, Karnataka, IN
2 Department of Pharmaceutical Analysis, Prin. K.M. Kundnani College of Pharmacy, Jote Joy Building, Cuffe Parade, Mumbai, IN

Abstract

A gradient LC method was developed for determination and quantitation of Sitagliptin in bulk drug and pharmaceutical formulations. The separation was accomplished on a symmetry reversed phase C18 column, 75 mm ×4.6mm I.D., 3.5μm column using mobile phase consist of acetonitrile and 0.03% formic acid at flow rate of 0.3ml/min. The eluents were monitored with a UV detector set at 268 nm as detection wavelength. The investigated validation elements showed the method has acceptable specificity, accuracy, linearity, precision, robustness. This method was found to be linear within range of 100 to 500 μg.ml^-1 (r=0.9999). The method could be of use for rapid and routine quality control analysis of Sitagliptin. Thus, the developed method can be used for process development as well as quality assurance of Sitagliptin in bulk drug and pharmaceutical formulations.

Keywords

Sitagliptin Phosphate, RP-HPLC, Validation, Liquid Chromatography, Quality Assurance.

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M. M. Maste ¹, M. C. Kalekar ¹, N. Kadian ¹, A. R. Bhat ¹

Affiliations
1 Department of Pharmaceutical Chemistry, KLEU’s College of Pharmacy, Belgaum, IN

Abstract

Present work describes a precise, accurate and reproducible Reverse phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous estimation of Ramipril and Amlodipine in tablet dosage form on Phenomenex C₁₈ column (245 mm × 4.5 mm, 5 μm, particle size) using acetonitrile: phosphate buffer, (60:40 v/v) as mobile phase at flow rate of 1.0 ml/min and the detection wavelength was 222 nm. The retention time for Ramipril and Amlodipine was found to be 3.41 and 6.10 min respectively. Proposed method was validated for precision, accuracy, linearity range, robustness and ruggedness.

Keywords

Amlodipine, Ramipril, RP-HPLC.

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M. M. Maste ¹, P. Jeyarani ¹, M. C. Kalekar ¹, A. R. Bhat ¹

Affiliations
1 Department of Pharmaceutical Chemistry, K. L.E.U’s College of Pharmacy, Belgaum, IN

Abstract

The present research work was aimed to synthesize some benzimidazole acetic acid derivatives and also the derivatives associated with 1,2,4 triazolone and was investigated for their biological activities. A two different series of compounds were synthesized, 4-[(2-aryl methyl- 1H-benzimidazol-1-yl) acetyl] 5 nitro-2,4 -dihydro-3H-1,2,4 triazol- 3-one and 4-[(2-aryl methyl-1H-benzimidazol-1-yl)methyl] 5 nitro-2,4- dihydro-3H-1,2,4 triazol-3-one. The newly synthesized compounds have been characterized by their analytical and spectral (IR and ¹H NMR, C¹³ NMR, mass spectra) properties. All the compounds have been screened for their anti tubercular and antimicrobial activities by standard methods. Results of activities reveal that compound shown promising anti tubercular activity at both the concentration compared to standard drug streptomycin, and shown good antimicrobial activities.

Keywords

1,2,4 Triazolone, Benzimidazole, Anti Tubercular, Anti Bacterial , Antifungal Activity.

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